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In Vitro Wound Healing

Preclinical Models of Wound Healing for Rapid Mechanistic Data

Epistem provides in vitro wound healing assays that quickly generate data on processes such as cell proliferation, migration, wound contraction and angiogenesis.

Why In Vitro Wound Models?

Rapid proof of concept data measuring effects on cell proliferation, migration, wound contraction and angiogenesis data. Assays may involve keratinocytes, fibroblasts or endothelial cells.

Scratch Assay

Monolayers of confluent cells are mechanically scratched and the rate at which the cells migrate to close the scratch is measured. The scratch is done by using an automated AutoScratch wound making tool (Agilent) which creates more consistent scratch wounds than manual methods, increasing reproducibility. Wound closure is followed by live imaging.

Wound Contraction Assay

Fibroblasts are seeded into collagen gels and the novel therapeutic agent applied. Contraction typically occurs over the following 5 days. Gels are imaged and the effect of a novel therapeutic agent on the rate of contraction determined.

Assays are performed using:

  • Cell lines
  • Primary human or murine keratinocytes
  • Primary human or murine fibroblasts

Primary cells can be obtained from diverse donor types, encompassing various ages and even other species.

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