In this industry-standard model, freshly isolated human keratinocytes are overlaid onto a human fibroblast-containing collagen gel and allowed to stratify.
Agents can be applied topically or mimic systemic exposure via the culture media.
Other cell types, such as melanocytes, can also be incorporated. The Epistem model is assembled and used in-house and allows for an extended assay period (up to 10 days).
The donor skin used to isolate each cell type can be tailored (e.g., ethnicity, age, body site).
The service is also supported by Epistem’s fully GCLP accredited histology and immunohistochemistry service.
Readouts
- Histometric analysis
- Proliferation, differentiation and apoptotic markers
- WST-1 assay for cytotoxicity screening
- UV damage marker quantification
- Protein expression
- Gene expression including anti-ageing and anti-oxidant pathways
