Epistem can assist in the selection and provision of appropriate cell lines and tumour types expressing a given target. Target expression responses pre/post treatment can be quantified by cell count/viability assays, immunohistochemistry with image analysis, flow cytometry or by gene expression profiling (including next generation sequencing).
Ideally, such studies will also test for potential side effects (toxicities), including the responses of normal cells/tissues that may express low levels of the target (or target family members).
Models include:
- Xenografted tumours
- Tissue biopsies
Readouts
- IC50 and EC50
- Viability, Proliferation and Apoptosis
- Histology
- Immunohistochemistry / Quantitative Image Analysis
- Flow cytometry
- Next Generation Sequencing
- PCR
