Antigen Presenting Cell Assay
The Antigen Presenting Cell (APC) assay utilises dendritic cells and macrophages to quickly generate data on the efficacy of your agent in modulating inflammatory responses. A pure APC population of bone marrow or monocyte-derived dendritic cells and macrophages are stimulated and the effects of a therapeutic agent on APC differentiation and activity are assessed.
T-Cell Assays
A service to evaluate the effectiveness of new therapeutic agents on T-cell activity and pathogenic T-cell populations. The assays are used to assess cytokine production and activation-induced cell death in T-cells isolated from peripheral blood and secondary lymphoid organs. Potential therapeutic agents are applied directly to naïve or activated T-cells.
Identification of Pathogenic T-cell Populations
Th1 and Th17 T-cells play a major role in autoimmune and inflammatory disease pathogenesis. Target pathogenic cell populations from inflamed tissue, peripheral blood and draining lymph nodes are isolated and identified by flow cytometry. The efficacy of novel therapeutic agents to modify these populations is assessed.
Readouts
- Flow cytometry
- Cytokine multiplex analysis
- Gene expression
- ELISA assay
