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Adoptive T-Cell Transfer-Mediated Colitis

Adoptive T-Cell Transfer Is a Widely Used Technique for Inducing Chronic Colitis in Preclinical Research Models

Our most popular IBD model, with many characteristics reflective of the human disease.

Why T-Cell Transfer?

This model is dependent on adaptive immunity and is suitable for the evaluation of therapies that can be administered either prophylactically or therapeutically

In this technique, naïve T-cells isolated from healthy donors are transferred into an immunodeficient recipient.  The unregulated activation of the donor T-cells by luminal antigens from the gut triggers an inflammatory response in the colon, resulting in the development of colitis.

This adoptive T-cell transfer model typically runs over 4-5 weeks. Colitis is associated with a Th1/Th17-type immune response and is characterised by epithelial hyperplasia, goblet cell depletion and infiltration of inflammatory cells into the mucosa and submucosa; crypt abscess formation and ulceration may also be observed.

Several options are available for the T-cell donor/recipient pairs, so that studies can be tailored to your specific needs.

Primary Readouts

  • Large bowel histopathology

Further Readouts

  • Large bowel weight : length ratio
  • Immunohistochemistry and immunofluorescence
  • Diarrhoea severity
  • Weight loss
  • FACS analysis of inflammatory cell response
  • Multiplex analysis of serum and tissue cytokines
  • Protein expression
  • Gene expression
  • Intestinal permeability assay (FITC Dextran)
  • ELISA: MPO & Fecal Lipocalin (Lcn-2) assay
  • T-Cell engraftment flow cytometry confirmation

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